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Section15:Data Types and Associated Rules for Enzymatic Assays: Inhibition Mode
From Assay Guidance Wiki
Normalized Results
Inhibition with a UOM of % based on complete enzyme inhibition (dynamic range of the assay).
Calculation:
Max = The observed enzyme activity measured in the presence of enzyme, substrate(s) and cofactors utilized in the method
Min = The observed enzyme activity measured in the presence of substrate(s) and cofactors utilized in the method, and (a) in the absence of enzyme, or (b) in the presence of a fully inhibited enzyme
Derived Results: Absolute IC50, Relative IC50
Relative IC50: the molar concentration at which 50% of maximal inhibition for that substance is observed.
Absolute IC50: the molar concentration of a substance that reduces the enzymatic activity to 50% of the total enzymatic activity.
Optional Result Types for Data Publication
The following tables include possible result types that can be used when calculating and publishing data from assays run in Inhibition Mode or Stimulation Mode.
| Enzyme | |
|---|---|
| Inhibition Mode | |
| SP | CRC |
| Inhibition | |
| Bottom | |
| Top | |
| Hill Coefficient | |
| Inhibition Max | |
| Inhibition at Maximum Tested Concentration | |
| Inhibition at Maximum Included Concentration | |
| Relative or Absolute IC50 | |
| Enzyme | |
|---|---|
| Stimultation Mode | |
| SP | CRC |
| Stimultation | |
| Bottom | |
| Top | |
| Hill Coefficient | |
| Stimulation Max | |
| Stimulation at Maximum Tested Concentration | |
| Stimulation at Maximum Included Concentration | |
| Relative EC50 | |
