Section5:Reagents

From Assay Guidance Wiki

Quality reagents are one of the most important factors involved in assay development. Validated reagents of sufficient quantity are critical for successful screen efforts over a long period of time. The primary reagents required for a radioactive receptor binding assay which are discussed on the following pages are:

• Receptor (membranes or purified)
• Radioligand

A section on methods of generating reagents for membrane binding assays can be found in the Appendix for the Receptor Binding Assays section of this handbook.