Section7:Example, Transient Transfection of Cells

From Assay Guidance Wiki

Various methods of transient transfection are described in the literature and they differ in their efficiency of transfection and cell toxicity. The primary decision for assay development involved the choice of a transfection method and reagent that yielded high efficiency, minimal toxicity and yet was compatible with the high cell volume requirements of HTS. Following is a list of transfection methods that can be tested to determine the optimal transfection protocol for each cell type. Measure the amount of toxicity, throughput and efficiency of transfection to determine the optimal method.